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KMID : 0379120060340010034
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Korean Journal of Mycology
2006 Volume.34 No. 1 p.34 ~ p.38
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Articles : Proton Beam Sensitivity of Basidiospore and Mycelium in Pleurotus ostreatus
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Kwon Hye-Jin
Kong Won-Sik
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Abstract
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To assess the effects of proton beam on radiation sensitivity of the basidiospore and mycelium of oyster mushroom (Pleurotus ostreatus), the $D_{10}$ values and $L_{50}$ (lethal 50%) values were analysed. By the proton beam radiation, the survival rate and germination rates increased at the dose of $10\;Gy{\sim}100\;Gy$ and then decreased significantly over 500 Gy. $L_{50}$ values of basidiospore and mycelium of Pleurotus ostreatus were over 500 Gy and 400 Gy, respectively. $D_{10}$ values were calculated from linear regression formulae ($D_{10}\;=\;-1/slope(b)$, y = a + bx) as 750 Gy and 1,250 Gy, respectively. Based on our experiment, the optimum dose of proton beam as a mutation source would be between from 500 Gy to 750 Gy for basidiospores and from 400 Gy to 1000 Gy for mycelium of oyster mushroom.
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KEYWORD
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Germination rate, Mutation, Oyster mushroom, Proton beam, Sensitivity
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